Immunolocalization of histone H3 isoforms in mitotic M. muntjak chromosomes. M. muntjak cells stained with antibodies against H3K27me1 (A) or H3K27me3 (B), or costained with H3K27me3 and H3S28p (C and D). H3K27me1 and H3K27me3 are distributed throughout chromosome arms, whereas H3S28p is more peripheral. Flattened z stacks are shown. DAPI, DNA stain. Bars, 10 µm. Images on the right show enlarged segments of the boxed regions (bars, 1.25 µm).