Figure 8.
Figure 8. Determinants of RhoBTB3 targeting to the Golgi. (A) Diagram of N-terminally Myc-tagged constructs; the Rho domain is shown in red and the BTB domain in blue. (B–K) Immunofluorescence microscopy of the localization of the indicated constructs in HeLa cells, detected with anti-Myc antibody. Bar, 20 µm. Insets show enlarged Golgi regions, identified using anti-GCC185 antibody. Bars (insets), 5 µm. (L) RhoBTB3 is a peripheral membrane protein. After 10 min on ice with the treatments indicated, membrane-associated or soluble fractions were obtained by centrifugation at 100,000 g and analyzed by immunoblot. Syntaxin 10 was used as an internal membrane control; GDI-β served as a cytosolic marker protein. Molecular mass marker mobility is shown at the left in kilodaltons.

Determinants of RhoBTB3 targeting to the Golgi. (A) Diagram of N-terminally Myc-tagged constructs; the Rho domain is shown in red and the BTB domain in blue. (B–K) Immunofluorescence microscopy of the localization of the indicated constructs in HeLa cells, detected with anti-Myc antibody. Bar, 20 µm. Insets show enlarged Golgi regions, identified using anti-GCC185 antibody. Bars (insets), 5 µm. (L) RhoBTB3 is a peripheral membrane protein. After 10 min on ice with the treatments indicated, membrane-associated or soluble fractions were obtained by centrifugation at 100,000 g and analyzed by immunoblot. Syntaxin 10 was used as an internal membrane control; GDI-β served as a cytosolic marker protein. Molecular mass marker mobility is shown at the left in kilodaltons.

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