Figure 5.
Figure 5. Analysis of nuclear transport in Nsp1p-depleted cells. (A) PMET3-NSP1 cells expressing the indicated NLS reporters were incubated with methionine (0–4 h) to repress NSP1 expression. Reporter location was analyzed by epifluorescence microscopy. (B) Poly-A mRNA was visualized under similar conditions using FISH analysis. (C) The specified temperature-sensitive mutants were grown at room temperature and then shifted to 37°C for the indicated times. Nup188-GFP localization was analyzed by epifluorescence microscopy. Bars, 5 µm.

Analysis of nuclear transport in Nsp1p-depleted cells. (A) PMET3-NSP1 cells expressing the indicated NLS reporters were incubated with methionine (0–4 h) to repress NSP1 expression. Reporter location was analyzed by epifluorescence microscopy. (B) Poly-A mRNA was visualized under similar conditions using FISH analysis. (C) The specified temperature-sensitive mutants were grown at room temperature and then shifted to 37°C for the indicated times. Nup188-GFP localization was analyzed by epifluorescence microscopy. Bars, 5 µm.

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