Antibody-induced dissociation of KLP10A and Patronin from spindles. (A and B) Live embryos expressing KLP10A-GFP (A) or Patronin-GFP (B, green in merged) injected with tubulin (red in merged). Bar, 5 µm. KLP10A-GFP (A and Video 1) localizes to spindle poles, kinetochores (from prometaphase to anaphase A) and faintly to cortices and spindle MTs. Patronin-GFP (B and Video 2) localizes to the mitotic spindle and interphase cytoplasm. (C) Anti-KLP10A or anti-Patronin antibody injections did not affect the integrity of spindle poles marked by centrosomin (CNN)-GFP. (D and E) Microinjection of anti-KLP10A (D) and anti-Patronin (E) dissociated their target antigens from spindles. Left images show KLP10A-GFP and Patronin-GFP alone, and right images show merged images of tubulin (red) and KLP10A-GFP or Patronin-GFP (green). Arrows show injection sites. WT, wild type.