Figure 6.
Figure 6. Rdi1-mediated cytosolic targeting of Cdc42 is essential for symmetry breaking without actin. (A) Polarization of GFP-Cdc42 in wild-type cells, rdi1Δ cells, rdi1Δ cells expressing CDC24ΔPB1 from the CDC24 promoter, and rdi1Δ bem1Δ cells in DMSO or LatA after release from G1 pheromone arrest. Experimental procedure was as described in Fig. 1 B. The plots show means from averaging three experiments, and error bars correspond to SEMs. More than 80 cells were counted per time point per experiment. (B) Maximum projections of representative cells from A. Bar, 5 µm. (C) Serial dilution of cells with the indicated genotypes from an overnight culture with an OD of 1 were plated on YPD media and grown at 23°C for 3 d.

Rdi1-mediated cytosolic targeting of Cdc42 is essential for symmetry breaking without actin. (A) Polarization of GFP-Cdc42 in wild-type cells, rdi1Δ cells, rdi1Δ cells expressing CDC24ΔPB1 from the CDC24 promoter, and rdi1Δ bem1Δ cells in DMSO or LatA after release from G1 pheromone arrest. Experimental procedure was as described in Fig. 1 B. The plots show means from averaging three experiments, and error bars correspond to SEMs. More than 80 cells were counted per time point per experiment. (B) Maximum projections of representative cells from A. Bar, 5 µm. (C) Serial dilution of cells with the indicated genotypes from an overnight culture with an OD of 1 were plated on YPD media and grown at 23°C for 3 d.

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