The Bem1–Cla4 interaction is not required for Cdc24 localization or symmetry breaking. (A) Polarization of mCherry-Cdc42 (left) and Cdc24^ΔPB1-GFP-Cla4 (right) in RSR1 BEM1 cells and in rsr1Δ bem1Δ cells in DMSO or LatA upon release from G1 pheromone arrest. Experimental procedure was as described in Fig. 1 B. The plots show means from averaging three experiments, and error bars correspond to SEMs. More than 80 cells were counted per time point per experiment. (B) Maximum projections of representative cells from A. (C) Polarization of GFP-Cdc42 in axl2Δ rax1Δ BEM1 cells, axl2Δ rax1Δ bem1Δ, and axl2Δ rax1Δ bem1^W192K cells, as in Fig. 1 B. (D) Maximum projections of representative polarized cells from C. (E) Maximum projections of representative cells with polarized Cdc24-GFP in axl2Δ rax1Δ bem1^W192K cells after pheromone arrest release in LatA or DMSO. (F) Quantification of Cdc24-GFP polarization in axl2Δ rax1Δ cells and in axl2Δ rax1Δ bem1^W192K cells, as in Fig. 1 K. For the comparison of peak values at 0°, P = 0.5. Plots show normalized profiles averaged over n > 17 cells. Error bars correspond to SEMs. Bars, 5 µm.