Figure 5.
Figure 5. Perturbation of formins activity altered the actin-myosin node dynamics. (A) 3D kymographs of actin node and myosin foci formation and aggregation. (A, left) 800 nM LatA. (A, right) 20 µM pretreatment of SMIFH2 for 24 h and then 800 nM LatA. Bars, 7 µm. (B and C) MSD analysis from imaging data shown in A, for one single experiment in each case. Data obtained from the experiments are plotted as individual dots. (D) MSD of the control and perturbed samples from HeLa JW cells (summarized in Table 1; error bars indicate SD). (E and F) Comparisons of MSD data from experiment results (red for MSD of actin nodes in the control sample, green for actin nodes in the SMIFH2 treated sample), drift-diffusion model fitting (solid lines), and pure diffusion model fitting (dashed lines). (G) RNAi silencing of mouse DAAM1 was confirmed by immunoblotting.

Perturbation of formins activity altered the actin-myosin node dynamics. (A) 3D kymographs of actin node and myosin foci formation and aggregation. (A, left) 800 nM LatA. (A, right) 20 µM pretreatment of SMIFH2 for 24 h and then 800 nM LatA. Bars, 7 µm. (B and C) MSD analysis from imaging data shown in A, for one single experiment in each case. Data obtained from the experiments are plotted as individual dots. (D) MSD of the control and perturbed samples from HeLa JW cells (summarized in Table 1; error bars indicate SD). (E and F) Comparisons of MSD data from experiment results (red for MSD of actin nodes in the control sample, green for actin nodes in the SMIFH2 treated sample), drift-diffusion model fitting (solid lines), and pure diffusion model fitting (dashed lines). (G) RNAi silencing of mouse DAAM1 was confirmed by immunoblotting.

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