PLP is required for centrosome inheritance but not ACD. (A) Projections of prophase WT and plp⁻ NBs. Active (arrows) and inactive (arrowheads) centrosomes are indicated. The adjacent boxes are magnifications of the indicated proteins showing the apical (top) and basal (bottom) centrioles. (B) Angle of centrosome separation at NEB from live γ-Tub analysis of WT and plp⁻ NBs: WT (n = 42) and plp⁻ (n = 46). The mean angle of centrosome separation is significantly different: P < 0.0001, two-tailed Student’s t test. The means ± SD are indicated in red, and the shaded area corresponds to <90°. (C) Interphase and mitotic MTOCs from WT and plp⁻ NBs. (D) WT and plp⁻ NBs showed no difference in proper spindle alignment along the apical (α-PKC, yellow arrowheads) and basal axis. (E) Some plp⁻ mitotic spindles are abnormal compared with WT. (F–H) Mitotic index (WT: n = 38/169; plp⁻: n = 32/150; F), cell cycle length (WT: n = 26; plp⁻: n = 39; G), and NB number from anterior and posterior optic lobe (stained for Mira; H) are all unaffected and displayed as means ± SD. Dashed circles, NBs. Bars: (main images) 5 µm; (insets) 1.5 µm.