PLP prevents the localization of Polo to the mother centrosome. (A and B) Live Polo-GFP in WT (A) and plp⁻ (B) NBs. Polo also localizes to kinetochores (open arrowheads) and the midbody ring (asterisks). Times are minutes and seconds relative to NEB (red boxes). (C–E) Projections of NBs showing Polo-GFP (C), Cnb-GFP (D), and PLP (E). Open arrowheads in E show nonspecific YFP signals. Quantification in NBs of Polo (C′) from WT (n = 16, 5 brains) and plp⁻ (n = 22, 5 brains), Cnb (D′) from WT (n = 20, 6 brains) and plp⁻ (n = 17, 7 brains), and PLP (E′) levels from PACT-GFP (n = 16, 7 brains), UAS>YFP-Cnb-PACT/+ (no GAL4; noninduced; n = 15, 6 brains), and UAS>YFP-Cnb-PACT/Act>GAL4 (induced; n = 11, 4 brains). Data shown are from a single representative experiment from two repeats. See Materials and methods for statistical analysis. Only plp⁻ cells containing two γ-Tub–positive centrosomes were selected for quantification of Cnb in D′. The α-PLP antibody is against the N terminus and does not detect the PACT constructs. In all panels, apical (arrows) and basal (arrowheads) centrosomes are indicated. Data are means ± SD. *, P < 0.01; **, P < 0.001; ***, P < 0.0001. The adjacent boxes (grayscale) are magnifications of the indicated proteins showing the apical (top) and basal (bottom) centrioles. Dashed circles, NBs. A, apical; B, basal. Bars: (main images) 5 µm; (insets) 1.5 µm.