PLP prevents the maturation of the mother centrosome. (A and B) Live GFP–γ-Tub in WT (A) and plp⁻ (B) NBs. Apical (arrows) and basal (arrowheads) centrosomes are enlarged and shown below. A basal location from −20 min WT is enlarged to indicate no detectable γ-Tub (dotted box). Times are minutes and seconds relative to NEB (red boxes). (C) Projections of interphase NBs showing anti–γ-Tub. (C′) Quantification is of γ-Tub levels at interphase apical (A) and basal (B) centrosomes from single NBs (WT: n = 16, 5 brains; plp⁻: n = 22, 6 brains). Data shown are from a single representative experiment from four repeats. See Materials and methods for statistical analysis. Data are means ± SD. *, P < 0.01. The adjacent boxes (grayscale) are magnifications of the indicated proteins showing the apical (top) and basal (bottom) centrioles. Dashed circles, NBs. Bars: (main images) 5 µm; (insets) 1.5 µm.