PLP is enriched on the mother centrosome in interphase. The indicated proteins (green) were detected by immunofluorescence or direct fluorescence in interphase NBs (dashed circles) counterstained for Asl to localize apical/daughter (arrows) and basal/mother (arrowheads) centrosomes. The adjacent boxes (grayscale) are magnifications of the indicated proteins showing the apical (top) and basal (bottom) centrioles. (A) Projections of NBs. Cnn, Centrosomin. (B) Quantification of AI. Each data point from the graph represents one measurement from a single cell; data were collected from five to nine NBs from three to six brains. The means ± SD are shown by the red bars and numbers below the graph. Absolute fold enrichment (A/B) is also indicated. See Materials and methods for details. (C) Projections of WT NBs showing PLP distribution on centrioles. Mitotic cells are marked by pH3 (green), and open arrowheads indicate centrosomes from other cells. GMCs, ganglion mother cells. Bars: (main images) 5 µm; (insets) 1.5 µm.