Figure 6.
![Figure 6. Translocation of PS to the cytosolic leaflet by a flippase is required for Gcs1 TGN/EE localization. (A) Gcs1(186–352)-GFP localization to TGN/EE is restored in drs2Δ cells expressing wild-type (WT) Drs2, but not the PS-deficient Drs2 [QQ → GA] mutant. An extra copy of WT Dnf1 failed to suppress the Gcs1(186–352)-GFP localization defect, but the Dnf1 N550S mutant that can flip PS restored Gcs1(186–352)-GFP TGN/EE localization. (B) Neither DRS2 nor DNF1 overexpression could restore Gcs1(186–352)-GFP TGN/EE localization in PS-deficient cho1Δ cells. Bars, 5 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/202/6/10.1083_jcb.201305094/6/jcb_201305094_fig6.jpeg?Expires=1773836899&Signature=HWFZ8OO7ClHgnyJpr12CDRM7Y7zO1eH~uIFcFYXerZlR39NPs0fDdIu6gRm9eZU6m~CqiP13OIcZVhFwn4a~qepHuAxpYgbDelJErgO7qtMxBRm~t8HajASs8PH4KM4DXitDfQQqFRNZsFnoHvXkFpxj-sIIql7d67Nw~Sgv-NBsC5fxMk06L8iuFW5~SJ3tmv1IEEP51QOcKx4VzTrMVS3X7mPDWErbPLSwmUI6mBcKLUD3sDvX7RfGM1nhA6APJk1vPFcnIhjzcIhug0AkOpEhPs6jSa-oEbijGNHd~nYhNnu1zXQKt12BN39j7RgefeMZwWlaF~XkcYzC2XJFcg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Translocation of PS to the cytosolic leaflet by a flippase is required for Gcs1 TGN/EE localization. (A) Gcs1(186–352)-GFP localization to TGN/EE is restored in drs2Δ cells expressing wild-type (WT) Drs2, but not the PS-deficient Drs2 [QQ → GA] mutant. An extra copy of WT Dnf1 failed to suppress the Gcs1(186–352)-GFP localization defect, but the Dnf1 N550S mutant that can flip PS restored Gcs1(186–352)-GFP TGN/EE localization. (B) Neither DRS2 nor DNF1 overexpression could restore Gcs1(186–352)-GFP TGN/EE localization in PS-deficient cho1Δ cells. Bars, 5 µm.
