Cdc14 is neither sufficient nor necessary to bypass the spindle checkpoint. (A and B) net1Δ PDS1-MYC18 (yAC1999) and cdc55Δ PDS1-MYC18 (yAC1896) cells were grown at 25°C in YEPD, synchronized in G1 with α-factor, and released in the presence of nocodazole. 80 min after the release, when 90% of the cells were budded, α-factor was added again. Samples were collected for Western blotting and FACS. The data shown are from a single representative experiment out of three repeats. (C and D) GAL1-MAD2 (3X) PDS1-MYC18 (yAC489), GAL1-MAD2 (3X) PDS1-MYC18 cdc27-5A cdc16-6A (yAC1675), GAL1-MAD2 (3X) PDS1-MYC18 cdc14-1 (yAC1768), and GAL1-MAD2 (3X) PDS1-MYC18 cdc15-2 (yAC1780) cells were grown in YEPR at 23°C. They were synchronized in G1 with α-factor and released into galactose. After 2 h, the cultures were shifted to 37°C and resupplemented with α-factor. Samples were collected for Western blotting (C) and IF (D). The data shown in D are from single representative experiments out of three repeats. For the experiments shown, n = 100.