Figure 5.
Figure 5. Single cell analysis of adapting cells. (A) CLB2-GFP TUB2-Cherry GAL1-MAD2 (3X) (yAC1732) cells were grown at 30°C in synthetic medium containing raffinose, and then were either shifted to galactose (adapting cells; top) or left in raffinose (cycling cells; bottom). We show Clb2-GFP accumulation signal in the nucleus after smoothing (see Materials and methods). Individual traces are synchronized at the time when Clb2 starts increasing. (B) Top left, degradation rate of Clb2; top right, expression rate, i.e., the rate of Clb2 accumulation; bottom right, adaptation time; bottom left, maxima of Clb2 fluorescence. See also Fig. S4, Materials and methods, and Video 1. The data shown are from a single representative experiment out of three repeats. For the experiment shown, n = 50. Cells were analyzed with the aid of the program phyloCell, written by G. Charvin (unpublished data).

Single cell analysis of adapting cells. (A) CLB2-GFP TUB2-Cherry GAL1-MAD2 (3X) (yAC1732) cells were grown at 30°C in synthetic medium containing raffinose, and then were either shifted to galactose (adapting cells; top) or left in raffinose (cycling cells; bottom). We show Clb2-GFP accumulation signal in the nucleus after smoothing (see Materials and methods). Individual traces are synchronized at the time when Clb2 starts increasing. (B) Top left, degradation rate of Clb2; top right, expression rate, i.e., the rate of Clb2 accumulation; bottom right, adaptation time; bottom left, maxima of Clb2 fluorescence. See also Fig. S4, Materials and methods, and Video 1. The data shown are from a single representative experiment out of three repeats. For the experiment shown, n = 50. Cells were analyzed with the aid of the program phyloCell, written by G. Charvin (unpublished data).

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