Cdh1 is responsible for Pds1 and Clb2 degradation upon strong Cdc28 inhibition. (A and B) GAL-SIC1 (yAC2025) and GAL-SIC1 cdh1Δ (yAC2023) cells were grown in either raffinose (A) or glucose (B) at 23°C. When in log phase, nocodazole was added to the media for 2.5 h and then cells were either supplemented with galactose (A) or kept in glucose (B). Samples were analyzed by Western blotting (top) and FACS (bottom). The data are from a single representative experiment out of three repeats.