Cdh1 is dispensable for the adaptation to the spindle checkpoint. (A) Cdc28/Clbs trigger a chain of events that both promote (green) and inhibit (red) transition to anaphase. (B–D) GAL1-MAD2 (3X) (yAC489) and GAL1-MAD2 (3X) cdc27-5A cdc16-6A (yAC1675) cells were synchronized in G1 with α-factor and released in galactose-containing medium. 2 h after the release, α-factor was re-added. Samples were taken for Western blotting (B and D) and IF analysis (IF; C) with anti-tubulin antibodies. The data shown in C are from a single representative experiment out of three repeats. For the experiment shown, n = 100. (E) GAL1-MAD2 (3X) (yAC489), GAL1-MAD2 (3X) cdh1Δ (yAC1738), and GAL1-MAD2 (3X) cdc27-5A cdc16-6A (yAC1675) cells were grown at 30°C in YEPR and shifted to galactose. Samples were taken every hour and the proportion of metaphase spindles was measured by IF. The data shown are from a single representative experiment out of three repeats (n = 100).