Roles of TCR- and LFA-1-dependent signaling and calcium flux in MTOC repositioning. (A) Grading system used in the MTOC repositioning assay. (B) Shown are the percent contributions of the four classes of MTOC repositioning for the indicated conjugates (see text). The n values are shown here and in following figures within the bar graph. (C) Bright-field image of a JB2.7 (LFA-1 minus) Jurkat engaged to a +SEE Raji cell (left) and a maximum projection over time of the GFP-Cen-2 signal in the JB2.7 cell (right; the JB2.7 cell is outlined in yellow except for its interface with the APC, which is in red). Bar, 10 µm.