MKLP2 is required for proper Kif2A distribution and stem body alignment. (A) Immunostaining of Aurora B (middle) and Kif2A (bottom) in control or MKLP2-depleted cells. (B) Line profiles of anti-Kif2A immunostaining along the central spindle in control and MKLP2-depleted cells. The mean ± SE of ≥36 cells from two independent experiments is shown. (C) Control and MKLP2-depleted cells expressing RacGAP1-GFP. Red arrows indicate misaligned stem bodies. Broken lines indicate cell boundaries. (D) The mean ± SE of three independent experiments is shown (≥62 cells were analyzed in each experiment). *, P < 0.05, t test. a.u., arbitrary unit. Bars, 5 µm.