Kif2A is involved in the depolymerization of central spindle MTs at their minus ends. (A and B) Shortening of central spindle MTs in control, but not Kif2A-depleted, cells expressing EGFP–α-tubulin after treatment with 200 ng/ml nocodazole (drug was added at time 0). The mean ± SE of at least six samples from two independent experiments is shown in B. (C) Scheme of the photobleaching assay. (D and E) EGFP–α-tubulin–expressing cells were treated with 1 µg/ml nocodazole immediately before image acquisition, and then, slit-shaped photobleach marks were introduced (red arrowheads). A time plot of the central spindle length and distance between two bleach marks. The mean ± SE of seven samples from three independent experiments is shown. Bars, 5 µm.