Proximity ligation analysis reveals an interaction between CLASP and DG in vivo. (A) Interaction between CLASP1 and βDG in epiblast cells revealed by using the Duolink proximity ligation assay. (B and C) CLASP1/Laminin and GM130/βDG are used as negative controls. (D) Laminin–βDG interaction is used as a positive control. Broken lines indicate the tissue outline (top, apical limit of the epiblast; bottom, apical limit of the endoderm). (E) In epiblast cells electroporated with CLASP1 MO, CLASP1–βDG interaction signals are greatly reduced (arrowheads in E1). Bars, 20 µm.