CLASP affects basal DG localization during EMT. (A) CLASP1 + CLASP2 MOs reduce basal DG localization. (B) Standard control MO does not affect DG subcellular localization. (C) CLASP2-ΔMΔC reduces basal DG localization. (D) 5mis-CLASP1 MO–expressing cells show no change in DG localization. Arrowheads indicate reduction of basal DG localization. (E) Percentage of cells showing a reduction in basal DG. CLASP1 + CLASP2 MOs cells: 77.9%, n = 86 cells from five embryos. 5mis CLASP1 control MO cells: 19.5%, n = 82 cells from three embryos. eGFP-expressing control cells: 19.4%, n = 93 cells from three embryos. eGFP-ΔMΔC expressing cells: 62.6%, n = 91 cells from four embryos. (F) eGFP-CLASP2γ–expressing cells retain strong DG expression in the medial streak. Arrows indicate mislocalization of DG in CLASP2γ–expressing cells. (G) Immunoprecipitation with either anti–β-DG antibodies or anti-HA antibodies from HEK 293 cells transfected with human DG and either HA-CLASP1α, HA-CLASP2γ, or mock treatment. Bars, 20 µm.