DG mediates basal membrane–BM interaction in epiblast cells. (A) β-DG localization in epiblast cells. (B and C) Electroporation with control 2A-eGFP or wild-type (WT)-DG-2A-eGFP expression vector. No obvious change in laminin expression is observed in control 2A-eGFP–expressing cells (B). WT-DG–receiving cells (C) show ectopic laminin expression (yellow arrows). (D and E) DG-specific MOs cause premature laminin breakdown (white arrows). (D) Translation blocking (TB) MO. (E) TB-MO + splicing blocking (SB) MO. ps, primitive streak. Arrowheads indicate the streak midline. (F) Percentage of cells showing laminin breakdown. TB-MO cells: 81.7%, n = 149 cells. TB-MO + SB-MO cells: 74.5%, n = 153 cells. 5-mis TB-MO: 9.7%, n = 165. 5mis TB-MO + SB-MO cells: 10.0%, n = 171. Bars, 20 µm.