hGAAP expression alters focal adhesion dynamics. (A and E) U2-OS cells overexpressing hGAAP (A) or transfected with siRNAs (E) were transfected with vinculin-GFP and seeded onto fibronectin-coated dishes. After 30 min, individual cells were imaged at 2-min intervals for 2 h. Representative frames are shown, with arrows highlighting a single typical adhesion for each cell line. Bars, 10 µm. (B–D and F–H) Summary results (means ± SEM, n = 6 cells, with 4–10 focal adhesions analyzed in each) show mean lifetimes of focal adhesions (B and F) and rate constants for their disassembly (C and G) and assembly (D and H). **, P < 0.01; ***, P < 0.001 (Student’s t test). FA, focal adhesion.