Normal ciliogenesis precedes loss of cilia in ependymal cells depleted of glycylases. (A) Scheme of the experiment. (B) Lateral ventricle walls with ependymal cells 3 d after electroporation (A), expressing either scramble or TTLL8-shRNA (blue or contours). Cilia were visualized with acetylated tubulin (6-11B-1) and basal bodies (20H5). Arrows, cilia; asterisks, basal bodies of GFP-positive (blue) cells. Bars, 10 µm. (C) Quantification of GFP-positive cells with motile cilia 3 d after electroporation (B). Analysis was done as in Fig. 4 D. No significant differences between control and TTLL8 shRNA were detected (Welch’s t test).