A subset of TTLL enzymes is expressed in adult mouse ependymal cells. In situ hybridization revealed the expression patterns of TTLL genes encoding glutamylases (A) or glycylases (B) in coronal brain sections (Cc, corpus callosum; Cx, cortex; LV, lateral ventricle; Str, striatum). Controls in Fig. S1, B and C. (C) TTLL3 expression visualized by X-Gal staining in the ttll3^−/− mouse. (D) Schematic representation of the right hemisphere with the green box indicating the localization of zoom images showing the ependymal layer in A–C. (E) Summary of expression analysis of the TTLL genes. Only weak (+) or strong (++) expression levels were considered specific. Bars (A–C): 10 µm.