CP190 and Mod(mdg4) enter bodies independently of one another, but Su(Hw) requires Mod(mdge4) in larval tissue. (A) Wing discs from CP190-RNAi larvae stressed with 250 mM NaCl and labeled with CP190 (left) or Mod(mdg4) (middle). Unstressed controls labeled with Mod(mdg4) are shown on the right. GFP marks Dcr-2⁺ knockdown cells, and the dashed lines demarcate the anterior–posterior axis of the wing disc. (B) Wing discs from two trans-heterozygous CP190 mutant larvae, CP190^H31-2/CP190^P11 (left) and CP190^4-1/CP190^P11 (middle) and a balanced control containing full-length CP190 (right) stressed with 250 mM NaCl and stained with CP190 and Mod(mdg4). Note that our CP190 antibody recognizes the CP190^4-1 isoform but not the CP190^H31-2 isoform. Domains of CP190 (BTB, Asp rich [Asp], microtubule binding [Cen], Zinc finger [Znf], and Glu rich [Glu]) present in each truncated allele are indicated by the colored lines. (C) Wing discs from null mod^u1 homozygotes (left) and balanced heterozygotes (middle) stressed with 250 mM NaCl and stained with Su(Hw) and CP190. (right) Mod staining verifies absence of protein in the mod^u1 mutant. Bars, 2 µm.