Insulator bodies localize to DAPI-less regions and associate transiently with the nuclear lamina. S2 cells treated with or without 250 mM NaCl and stained for CP190 and lamin. (A) Bodies localized to the interior form in DAPI-less lacunas (white arrowheads). Intensity correlation analysis (boxed regions and insets) reveals regions of high overlap (gold) between CP190 bodies in the nuclear periphery and lamin. (B) Serial 1-µm z slices through a S2 nucleus stressed with 250 mM NaCl stained for CP190 and nuclear pore complex components (NUPs). (C) Nuclear halos generated from 250 mM NaCl-stressed S2 cells showing a highly extracted nucleus with no CP190 signal (yellow asterisks) and a less efficiently extracted nucleus (white asterisks) showing remnants of CP190 bodies colocalized with lamin. Bars: (A, main images) 2 µm; (A, insets) 0.5 µm; (B) 1 µm; (C) 4 µm.