SNPH dominantly immobilizes axonal mitochondria. (A and B) Kymographs (A) and quantitative analysis (B) showing axonal mitochondrial motility in snph^+/+ neurons expressing SNPH, Miro1, or Trak2 alone or coexpressing Miro1 + SNPH or Trak2 + SNPH. Hippocampal neurons were transfected at DIV9 and imaged at DIV12. Data were quantified from numbers of neurons (indicated in parentheses) in three independent experiments. Bars, 10 µm. Error bars show SEM. Student’s t test. Also see Fig. S2 and Videos 6 and 7.