Polo activity promotes the cytoplasmic localization of Gwl in prophase. (A) Phosphorylation of Gwl in its central region depends on Polo. (B) Increasing Polo activity induces a more cytoplasmic localization of Gwl in syncytial embryos. P values are from a paired t test. (C) Increasing Polo activity induces a more cytoplasmic localization of Gwl in cultured cells. Bars, 5 µm. (D) Time-lapse microscopy of D-Mel cells expressing GFP-Gwl and H2Av-mRFP. In control, DMSO-treated cells, GFP-Gwl is excluded from the nucleus in prophase (red arrowhead). In cells treated with BI2536 (50 nM) to inhibit Polo, GFP-Gwl is never excluded from the nucleus and becomes diffuse throughout the cell in mitosis. T0 was set as the time when cytoplasmic GFP-Gwl first begins to appear. Bars, 5 µm.
