Figure 2.
![Figure 2. Gwl contains two essential NLS motifs in its central region. (A) The central region of Gwl is necessary and sufficient for nuclear localization. D-Mel cells expressing the indicated truncations of Gwl tagged with Myc were examined by immunofluorescence on a confocal microscope. Identified NLS motifs are shown. Bar, 5 µm. (B and C) Mutation of both NLSs (NLSmut = K518M, K520M [in NLS1] + K564M, R566L [in NLS2; see Fig. S2]) prevents the nuclear localization of Gwl. Immunofluorescence in D-Mel cells (B) and in syncytial embryos (C) expressing the indicated proteins. Bars, 10 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/202/2/10.1083_jcb.201211141/6/jcb_201211141_fig2.jpeg?Expires=1771612413&Signature=ivNXMh~1lRnYo~dlRCHjGqt-u5jBEyk22HfCiw9LcvywL2VmOnEtSbjMBR-UDCRTGp1LM9lrNzZYfj6EGkodkp1N~IK8sEM4T5mlK~pXKulFkTaUWj44kULfeijbpYd5IFTv0MaW7bIRR61k9T~5oXrFg3XgDff7CYMTXD2GJUNAOvKVR-LKTM74V5YdhqtyKHBvJt87MSA1vrcgFAqRPOodKBHVBugHW0CfrYj9uzJ7cf8vHnublkMwBUC1XN3ZXMJuMVEnu6GQBzWsVBTAXT6dgM6qTZZhUx9jtEk0fd2zmtiXsX---SMXiCu6ccwoWqQbn0G2VcfXPv5CfdGizA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Gwl contains two essential NLS motifs in its central region. (A) The central region of Gwl is necessary and sufficient for nuclear localization. D-Mel cells expressing the indicated truncations of Gwl tagged with Myc were examined by immunofluorescence on a confocal microscope. Identified NLS motifs are shown. Bar, 5 µm. (B and C) Mutation of both NLSs (NLSmut = K518M, K520M [in NLS1] + K564M, R566L [in NLS2; see Fig. S2]) prevents the nuclear localization of Gwl. Immunofluorescence in D-Mel cells (B) and in syncytial embryos (C) expressing the indicated proteins. Bars, 10 µm.
