MT branching from the kMTs and non-kMTs. (A–C) Models showing examples of the “branching” sites on mother MTs. Mother, daughter, and granddaughter MTs are colored green, orange, and aqua, respectively. Closed ends, chromosome-facing open ends, and the sites of end-links are shown with magenta, white, and red spheres, respectively. The surface of the chromosomes is outlined in blue. The centriole pair is colored gray. Note that both kMTs and non-kMTs served as mother MTs (A), and in one case, a mother MT was connected by the end-links to two daughter MTs (B), and a daughter MT was further end-linked to a granddaughter MT (C). Arrows indicate the branching sites. Bars: (A and B) 1 µm; (C) 0.5 µm.