Figure 1.
Figure 1. Vacuole membrane proteins segregate into domains in Stat-phase. (A) Cells expressing Vph1-GFP, in Stat-phase, were visualized live at room temperature by fluorescence microscopy focusing on either the top or middle of the vacuole. Three types of microdomains are shown: partial, quasi-symmetrical, and coalesced. (B) Cells expressing Vph1-GFP were grown in SC medium. The percentage of cells with the indicated vacuolar domain patterns and the OD600nm (black line) were determined (n = 100–300 cells/time). (C) Cells expressing Vph1-GFP and Ivy1-mCherry, in Stat-phase, were visualized by fluorescent microscopy. Bars, 5 µm.

Vacuole membrane proteins segregate into domains in Stat-phase. (A) Cells expressing Vph1-GFP, in Stat-phase, were visualized live at room temperature by fluorescence microscopy focusing on either the top or middle of the vacuole. Three types of microdomains are shown: partial, quasi-symmetrical, and coalesced. (B) Cells expressing Vph1-GFP were grown in SC medium. The percentage of cells with the indicated vacuolar domain patterns and the OD600nm (black line) were determined (n = 100–300 cells/time). (C) Cells expressing Vph1-GFP and Ivy1-mCherry, in Stat-phase, were visualized by fluorescent microscopy. Bars, 5 µm.

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