Lymphatic vessels were detected in skull periosteum, suture, and dura. (A) Confocal imaging of coronal suture sections from 2-mo-old Prox1;Ai14 mice stained with Lyve1 (red). DAPI stains nuclei. Right panels are enlargements of white boxed regions in A. Scale bars: 200 µm. (B) Confocal imaging of non-sutural skull bone regions stained with Lyve1 (green) from Prox1;Ai14 (red) mice. Lyve1⁺;Prox1⁺ lymphatic vessels are localized to the periosteum and dura mater but absent from the bone. White arrowheads indicate double-positive structures. Scale bar: 200 µm.