NK cells reduced the viral load on DT-treated CCL3-EASER mice. (A) Scheme of the experiment. CCL3-EASER mice received 25 ng/g/BW of DT or PBS i.p. on two consecutive days to delete Ccl3-producing cells. 1 day later, leukocytes of the indicated organs were analyzed by flow cytometry. (B) Count of the indicated cell populations in liver, spleen, and lung. (C) Scheme of the experiment. After DT or PBS administration, mice were infected i.v. with mCMV and were partially reconstituted with CD45.1 splenic NK cells. Mice were analyzed 4 days after infection. (D) Representative contour plots indicating the presence of CD45.1 NK cells in the liver after adoptive cell transfer 4 days after infection. (E) Viral load of control or depleted CCL3-EASER mice 4 days after mCMV infection, from the same organs analyzed in D. Data is presented as means ± SD; n = 4 mice per group. Data is representative of two independent experiments. Unpaired nonparametric Mann–Whitney U-test was used: ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.