CCL3-EASER identified NK cells as the major producers of Ccl3. BL6 and CCL3-EASER mice were infected with 5 × 10⁵ PFU of mCMV and analyzed at 0, 4, 8, 16, and 48 h after infection (n = 4 mice per time point). (A) Representative overlapped dot plots of the indicated cell populations of liver-associated leukocytes from naive BL6 (black) and naive CCL3-EASER (red) mice. (B) Frequency of the indicated cell populations from total Ccl3-tdTomato⁺ cells in spleen, liver, and lung from naive EASER mice. Calculated as the percentage within tdTomato positive population. (C) Representative overlapped dot plots of the indicated cell populations of liver-associated leukocytes from mCMV infected BL6 (black) and infected CCL3-EASER (red) mice. (D) Frequency of the indicated cell populations from total Ccl3-tdTomato⁺ cells from spleen, liver, and lung before and after mCMV infection, calculated as in B. Data is presented as means ± SD and is representative of four mice per group for two independent experiments. DC, dendritic cells.