Figure S2.
STX11 in T and B cells. Mouse: (A) Efficiency of CD8 T cell depletion showing body weight over time (left) and frequency of CD8 T cells in the blood d4 p.i. and d12 in the spleen p.i. Related to Fig. 2 A. (B and C) Representative spleen sections d14 p.i. with VV (B) or VSV (C) in Stx11−/−, Stx11+/−, and Stx11+/+ mice. Scale bar 100 µm, B220 (B cell area) in green and PNA (GC-B cells) in red. (D) Western blot (5 µg total protein) of STX11 expression in naive and stimulated purified splenic CD4 T cells and B cells from WT (Stx11+/+) and Stx11−/− mice. Molecular mass is shown in kilodaltons. Human: (E) Gating strategy for Tfh cells; related to Fig. 3 A. Mouse: (F) CD4 T cell percentages and frequencies in spleen d6 (Stx11−/−n = 6, Prf1−/−n = 6) and d8 (Stx11−/−n = 6, Prf1−/−n = 5) p.i. with 200 PFU LCMV; each n = 2 independent experiments. (G) Correlation between frequencies of transferred SMARTA.WT into Stx11−/− (n = 8) mice and GC B cell frequencies after d12 p.i. with 200 PFU LCMV of n = 2 independent experiments. (A–G) Mean and SEMs are shown of Mann–Whitney U test, depending on normal distribution; ns indicates not significant. Source data are available for this figure: SourceData FS2.

STX11 in T and B cells. Mouse: (A) Efficiency of CD8 T cell depletion showing body weight over time (left) and frequency of CD8 T cells in the blood d4 p.i. and d12 in the spleen p.i. Related to Fig. 2 A. (B and C) Representative spleen sections d14 p.i. with VV (B) or VSV (C) in Stx11−/−, Stx11+/−, and Stx11+/+ mice. Scale bar 100 µm, B220 (B cell area) in green and PNA (GC-B cells) in red. (D) Western blot (5 µg total protein) of STX11 expression in naive and stimulated purified splenic CD4 T cells and B cells from WT (Stx11+/+) and Stx11−/− mice. Molecular mass is shown in kilodaltons. Human: (E) Gating strategy for Tfh cells; related to Fig. 3 A. Mouse: (F) CD4 T cell percentages and frequencies in spleen d6 (Stx11−/−n = 6, Prf1−/−n = 6) and d8 (Stx11−/−n = 6, Prf1−/−n = 5) p.i. with 200 PFU LCMV; each n = 2 independent experiments. (G) Correlation between frequencies of transferred SMARTA.WT into Stx11−/− (n = 8) mice and GC B cell frequencies after d12 p.i. with 200 PFU LCMV of n = 2 independent experiments. (A–G) Mean and SEMs are shown of Mann–Whitney U test, depending on normal distribution; ns indicates not significant. Source data are available for this figure: SourceData FS2.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal