Anti-PD-1 treatment has little impact on DC phenotype in the tumor-draining lymph node. (A and B) C57BL/6 mice were injected s.c. with MC38-OVA tumor cells (0.5.10⁶ cells). After 10 days, mice were treated with anti-PD-1 or an isotype control (250 µg, i.v.). On day 1 and day 3, lymph node DC phenotype was assessed by flow cytometry. Frequency (A) and phenotype (B) of migratory DCs (mDC, CD11c^int, MHC class II^high) and conventional DC (cDC, CD11c^high, MHC class II^int). Representative of two independent experiments. Statistical analyses were performed using two-way ANOVA. *, P < 0.05; **, P < 0.01.