Figure S4.
The identification of the interaction between BCL2L13 and ARL6IP1 in regulating autophagosome formation. (A) Schematic representation of protein microarray with >21,000 human proteins (HuProt v3.1 protein array). (B) Schematic diagram of protein microarray data analysis and filtering method involving ARL6IP1 protein. (C) Classification of BCL2 family members based on domain organization predicted to bind with ARL6IP1. BH; BCL2 homology domains, TM; transmembrane domains. (D) Quantifying the interaction affinity score between ARL6IP1 and BCL2 family genes using an array. (E) The protein level of ARL6IP1 and omegasome-related genes in ARL6IP1 KO and WT MEFs after HBSS starvation for 1 h. (F) Immunofluorescence colocalization of LC3B and WIPI2 from ARL6IP1 KO and WT MEFs after HBSS starvation. Experiments (E and F) were performed on two independent biological repeats. Source data are available for this figure: SourceData FS4.

The identification of the interaction between BCL2L13 and ARL6IP1 in regulating autophagosome formation. (A) Schematic representation of protein microarray with >21,000 human proteins (HuProt v3.1 protein array). (B) Schematic diagram of protein microarray data analysis and filtering method involving ARL6IP1 protein. (C) Classification of BCL2 family members based on domain organization predicted to bind with ARL6IP1. BH; BCL2 homology domains, TM; transmembrane domains. (D) Quantifying the interaction affinity score between ARL6IP1 and BCL2 family genes using an array. (E) The protein level of ARL6IP1 and omegasome-related genes in ARL6IP1 KO and WT MEFs after HBSS starvation for 1 h. (F) Immunofluorescence colocalization of LC3B and WIPI2 from ARL6IP1 KO and WT MEFs after HBSS starvation. Experiments (E and F) were performed on two independent biological repeats. Source data are available for this figure: SourceData FS4.

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