Figure S1.
Information of Arl6ip1−/−mice. (A) The WT allele of Arl6ip1 (top) and the targeted locus of Arl6ip1 (bottom) is shown in the schematic diagram. The L1L2_Bact_P cassette was inserted at position 118127554 of Chromosome 7 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human β-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 118125888. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065). Gray boxes represent exons and black lines represent introns. (B) Mouse genotyping by PCR in WT (Arl6ip1+/+), heterozygous (Arl6ip1+/−), and homozygous Arl6ip1 KO (Arl6ip1−/−) mice. (C) RT-PCR (left) and real-time PCR (right) analysis to confirm the loss of mRNA level in Arl6ip1−/− brain tissues. Arl6ip1+/+ was used as a positive control for evaluating the ARL6IP1 expression (male and female mice aged 9 mo, total n = 4/group). (D) The protein level of ARL6IP1 in brain tissue lysates from Arl6ip1−/− and Arl6ip1+/+ mice. (E) Immunofluorescence image of ARL6IP1 level in the brain tissues from Arl6ip1−/− and Arl6ip1+/+ mice at 6 mo (20×; scale bars: 1 mm). Data are represented by three independent biological replicates, the mean and error bars represent the SEM, and the two-tailed unpaired t test. **P < 0.01. CTX, cortex; HIPP, hippocampus; THs, thalamus; HY, hypothalamus; MB, midbrain; Po, pons; CB, cerebellum. Source data are available for this figure: SourceData FS1.

Information of Arl6ip1 −/− mice. (A) The WT allele of Arl6ip1 (top) and the targeted locus of Arl6ip1 (bottom) is shown in the schematic diagram. The L1L2_Bact_P cassette was inserted at position 118127554 of Chromosome 7 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human β-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 118125888. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065). Gray boxes represent exons and black lines represent introns. (B) Mouse genotyping by PCR in WT (Arl6ip1+/+), heterozygous (Arl6ip1+/−), and homozygous Arl6ip1 KO (Arl6ip1−/−) mice. (C) RT-PCR (left) and real-time PCR (right) analysis to confirm the loss of mRNA level in Arl6ip1−/− brain tissues. Arl6ip1+/+ was used as a positive control for evaluating the ARL6IP1 expression (male and female mice aged 9 mo, total n = 4/group). (D) The protein level of ARL6IP1 in brain tissue lysates from Arl6ip1−/− and Arl6ip1+/+ mice. (E) Immunofluorescence image of ARL6IP1 level in the brain tissues from Arl6ip1−/− and Arl6ip1+/+ mice at 6 mo (20×; scale bars: 1 mm). Data are represented by three independent biological replicates, the mean and error bars represent the SEM, and the two-tailed unpaired t test. **P < 0.01. CTX, cortex; HIPP, hippocampus; THs, thalamus; HY, hypothalamus; MB, midbrain; Po, pons; CB, cerebellum. Source data are available for this figure: SourceData FS1.

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