Accumulation of oligodendrocyte-lineage cell-derived ribosomal reporter Rpl10a-EGFP in neurons. (A) Ribosomal Rpl10a-EGFP reporter in oligodendrocyte-lineage cells (arrows) and neurons in the cortex, thalamus, striatum, and corpus callosum of adult PDGFRα-Cre:Rpl10a-EGFP mice. The region of the yellow box is enlarged and shown as a single plane image with orthogonal projection. (B) In adult PDGFRα-Cre:EYFP mice EYFP reporter protein was found only in oligodendrocyte-lineage cells (arrows) and not in neurons. (C and D) Quantification of reporter-positive NeuN⁺ neurons and Olig2⁺ oligodendrocyte-lineage cells in the cortex, thalamus, and striatum of adult PDGFRα-Cre:Rpl10a-EGFP and PDGFRα-Cre:EYFP mice. (E and F)Sox10-Cre mice carrying the Rpl10a-EGFP or Rpl10a-EGFP H2B-mCherry reporter allele had a distinct craniofacial phenotype, displaying malocclusion and runty appearance compared to Cre-negative siblings carrying the reporter allele. All data are presented as mean ± SEM. Each circle represents an individual animal. Data are representative of two (A–D) and four (E and F) independent experiments. P values were determined by two-way ANOVA with Bonferroni post hoc test. ****, P < 0.0001. Scale bars: 20 µm for A and B; 5 µm for enlargements in A. CC, corpus callosum; Cx, cortex; Stri, striatum; Thal, thalamus.