CNS neurons receive nuclear and ribosomal material from SOL. (A) 1 ng of DT was injected into the cerebral cortex or thalamus of 8–12-wk-old Sox10-Cre:iDTR Sun1-sfGFP and control Sox10-Cre:Sun1-sfGFP mice. (B) Selective ablation of oligodendrocyte-lineage cells at the injection site in Sox10-Cre:iDTR Sun1-sfGFP mice 3 d after DT injection. Neuronal density at the injection site in Sox10-Cre:iDTR Sun1-sfGFP mice was indistinguishable from neuronal density in control Sox10-Cre:Sun1-sfGFP mice. (C–E) Quantification of Olig2⁺ oligodendrocyte-lineage cells, NeuN⁺ and NeuN⁺GFP⁺ neurons in the cortex or thalamus 3 d after DT injection. (F) Quantification of GFP MFI in individual neurons in the cortex or thalamus 3 d after DT injection. (G–J) Nuclear and ribosomal reporters in oligodendrocyte-lineage cells (arrows) and selective neurons (arrowheads) in the cortex of adult Sox10-Cre:H2B-mCherry, Sox10-Cre:Rpl22-HA, Sox10-Cre:Rpl10a-EGFP H2B-mCherry, Sox10-Cre:Rpl10a-EGFP RanGAP1-mCherry mice. The region of the yellow box is enlarged and shown as a single plane image with orthogonal projection. All data are presented as mean ± SEM. Each circle represents an individual animal in C–E and individual cell in F. Data are representative of two (G–J) and three (B–F) independent experiments. P values were determined by two-way ANOVA with Bonferroni post hoc test. ns, P > 0.05; **, P < 0.01; ***, P < 0.001. Scale bars: 100 µm for B; 20 µm for G–J and 5 µm for the enlargements in G–J. A.U., arbitrary units; CC, corpus callosum; Cx, cortex; HA, hemagglutinin; Hy, Hypothalamus; ns, not significant; Thal, thalamus.