SOL-derived ribosomal material accumulates in neurons throughout the entire CNS. (A) Sagittal view showing distribution of reporter-positive neurons in the brain of adult Sox10-Cre:Rpl10a-EGFP mice. (B) Reporter-positive Purkinje neurons (arrows) were found in the cerebellum, while most of NeuN⁻ neurons in the molecular layer (ML) and NeuN⁺ neurons in the granular layer (GL) of the cerebellum were reporter-negative. (C) Reporter-positive pyramidal neurons (arrows) in the gray matter of the spinal cord in adult Sox10-Cre:Rpl10a-EGFP mice. (D) No Rpl10a-EGFP immunoreactivity was detected in the CNS of Cre-negative siblings carrying the Rpl10a-EGFP transgene. (E) Fluorescence-activated nuclei sorting analysis of NeuN⁺GFP⁺ nuclei isolated from the cortex or thalamus of adult Sox10-Cre:Rpl10a-EGFP mice. Gating for NeuN⁺ population was defined using fluorescence minus one (FMO) control. (F) Quantification of NeuN⁺GFP⁺ nuclei isolated from the cortex or thalamus. (G) No reporter-positive astrocytes (GFAP⁺) or microglia (Iba1⁺) were detected in the cortex of Sox10-Cre:Rpl10a-EGFP mice. Some reporter-positive astrocytes (arrows) were found in the hippocampal stratum lacunosum-moleculare (SLM), striatum and thalamus. No reporter-positive microglia were found in the hippocampus, striatum or thalamus. All data are presented as mean ± SEM. Each circle represents an individual animal. Data are representative of two (G) and three (A–F) independent experiments. P value was determined by unpaired t test. *, P < 0.05. Scale bars: 1,000 µm for A; 100 µm for C and D; 50 µm for B and G. CE, cerebellum; CP, caudate putamen, Cx, cortex; Hip, hippocampus; Hy, hypothalamus; LV, lateral ventricle; MB, midbrain; Me, medulla; OB, olfactory bulb; PCL, Purkinje cell layer; Th, thalamus.