Figure 7. Biofilm prevents the... | Rockefeller University Press

Figure 7.

Biofilm prevents the infiltration of neutrophils into bacterial lesions. WT or D1−/−D1L3−/− DKO mice were infected i.v. with 1 × 107 CFU of S. aureus, and their kidneys were examined by immunohistochemistry 72 h later. (A–D) Sections of WT (A and B) and DKO (C and D) mice were stained with DAPI and antibodies to S. aureus and Ly-6G. Shown are low-magnification images of the entire kidney (A and C; scale bar, 1 mm) and magnified images of areas in white boxes (B and D; scale bars, 50 μm). (E and F) Quantification of total Ly-6G–positive area (neutrophil infiltration; E) and Ly-6G–negative area within DAPI-positive lesions (neutrophil exclusion from lesions; F) in kidney sections. Symbols represent individual mice; bars represent the median. Statistical significance was determined by Mann–Whitney test; two-tailed P value < 0.001 (***).

Biofilm prevents the infiltration of neutrophils into bacterial lesions. WT or D1^−/−D1L3^−/− DKO mice were infected i.v. with 1 × 10⁷ CFU of S. aureus, and their kidneys were examined by immunohistochemistry 72 h later. (A–D) Sections of WT (A and B) and DKO (C and D) mice were stained with DAPI and antibodies to S. aureus and Ly-6G. Shown are low-magnification images of the entire kidney (A and C; scale bar, 1 mm) and magnified images of areas in white boxes (B and D; scale bars, 50 μm). (E and F) Quantification of total Ly-6G–positive area (neutrophil infiltration; E) and Ly-6G–negative area within DAPI-positive lesions (neutrophil exclusion from lesions; F) in kidney sections. Symbols represent individual mice; bars represent the median. Statistical significance was determined by Mann–Whitney test; two-tailed P value < 0.001 (***).