Figure S3. Induction of neutrophilia by... | Rockefeller University Press

Figure S3.

$Induction of neutrophilia by S. aureus infection and sterile inflammation. (A–C) WT or D1−/−D1L3−/− mice were given three doses of LPS and one dose of heat-killed E. coli and sacrificed 24 h later. Shown is the fraction of blood neutrophils measured by CBC (A) and the mean fluorescent intensity (MFI) of ICAM-1 (B) and Sca-1 (C) on the indicated cell types in the peripheral blood measured by flow cytometry at baseline (pre) and 24 h after E. coli injection. Symbols represent individual mice; bars represent mean ± SEM; dotted lines represent the range of normal values for CBC. (D–H) D1−/−D1L3−/− mice were treated as above or infected with 1 × 107 CFU of S. aureus and sacrificed 18 or 72 h after infection (hpi). Shown is the fraction of neutrophils in the peripheral blood measured by CBC (D); spleen weight (E); and red blood cell count (F), hemoglobin concentration (G), and platelet count (H) in the peripheral blood measured by CBC. Symbols represent individual mice; bars represent mean ± SEM; dotted lines represent the range of normal values. Statistical significance was determined by Wilcoxon matched-pairs signed rank test (A–C) and two-way ANOVA followed by Tukey’s multiple-comparison test (D–H). Two-tailed P values < 0.05 (*), <0.01 (**), <0.001 (***), and <0.0001 (****).$

Induction of neutrophilia by S. aureus infection and sterile inflammation. (A–C) WT or D1^−/−D1L3^−/− mice were given three doses of LPS and one dose of heat-killed E. coli and sacrificed 24 h later. Shown is the fraction of blood neutrophils measured by CBC (A) and the mean fluorescent intensity (MFI) of ICAM-1 (B) and Sca-1 (C) on the indicated cell types in the peripheral blood measured by flow cytometry at baseline (pre) and 24 h after E. coli injection. Symbols represent individual mice; bars represent mean ± SEM; dotted lines represent the range of normal values for CBC. (D–H) D1^−/−D1L3^−/− mice were treated as above or infected with 1 × 10⁷ CFU of S. aureus and sacrificed 18 or 72 h after infection (hpi). Shown is the fraction of neutrophils in the peripheral blood measured by CBC (D); spleen weight (E); and red blood cell count (F), hemoglobin concentration (G), and platelet count (H) in the peripheral blood measured by CBC. Symbols represent individual mice; bars represent mean ± SEM; dotted lines represent the range of normal values. Statistical significance was determined by Wilcoxon matched-pairs signed rank test (A–C) and two-way ANOVA followed by Tukey’s multiple-comparison test (D–H). Two-tailed P values < 0.05 (*), <0.01 (**), <0.001 (***), and <0.0001 (****).