Inflammatory Th subsets in ZAC mice by single-cell transcriptomic analysis. (A) UMAP visualization of splenic Foxp3⁻CD4⁺ Tconv and Foxp3⁺ Treg cells from WT or ZAC mice (two mice each) showing gene expression-based clusters by scRNA-seq. (B) Composition of each UMAP cluster by Tconv or Treg cells from WT or ZAC mice. (C) Dot plots showing expression levels of Th and Treg signature genes, and composition of WT and ZAC samples in each cluster. (D) Expression levels of Th and Treg signature genes.