Figure S4.
Supplementary immunoblots for Figs. 1, 2, and 4. (A and B) N/TERT-1C1C-EGFP or monoclonal NLRP1 knockout N/TERT-1C1C-EGFP cells were stimulated with DMSO, 30 μM Tal (A), 15 µM Aniso (A), UV for 3 min (A, B), 2 µM Lacti (B), or infected with SFV at an MOI of 5 (B) for 20 h. Stimulation was performed in the presence or absence of 10 µM Dora. Lysates and precipitated supernatants were analyzed by immunoblot with antibodies for NLRP1, caspase-3, PARP, caspase-1, IL-1β, and Vinculin. Please note that the order of samples is different for lysates and supernatants in B. Excerpts of these immunoblots are displayed in Figs. 2 and 4. Data displays experiment representatives of two independent experiments. MW, molecular weight. Source data are available for this figure: SourceData FS4.

Supplementary immunoblots for Figs. 1, 2, and 4. (A and B) N/TERT-1C1C-EGFP or monoclonal NLRP1 knockout N/TERT-1C1C-EGFP cells were stimulated with DMSO, 30 μM Tal (A), 15 µM Aniso (A), UV for 3 min (A, B), 2 µM Lacti (B), or infected with SFV at an MOI of 5 (B) for 20 h. Stimulation was performed in the presence or absence of 10 µM Dora. Lysates and precipitated supernatants were analyzed by immunoblot with antibodies for NLRP1, caspase-3, PARP, caspase-1, IL-1β, and Vinculin. Please note that the order of samples is different for lysates and supernatants in B. Excerpts of these immunoblots are displayed in Figs. 2 and 4. Data displays experiment representatives of two independent experiments. MW, molecular weight. Source data are available for this figure: SourceData FS4.

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