Figure 3.
MLV drainage from the caudal CAV. (A) Schematic of venous sinuses, veins, and internal carotid artery at the base of the brain. (B–I) Dashed lines indicate the level of coronal sections in B–I (inset in A), and lymphatics are numbered 1–3. BS, brainstem; bv, basilar vein; ica, internal carotid artery; ICAV, inter-CAV; ijv, internal jugular vein; IPETS, inferior petrosal sinus; iptgv, interpterygoid emissary vein; OB, olfactory bulb; pbv, posterior basal vein; pfv, posterior facial vein; SPETS, superior petrosal sinus. (B) Coronal view of the right CAV. OVA-A555 was injected into the Th-Lb spine 45 min before sacrifice, and the sample was labeled with antibodies recognizing blood endothelium markers CD31 and PDLX that label pituitary gland (PG) vessels as well as dura mater veins and sinuses (blue arrowheads). Blue dashed line, limits of CAV and iCAV. Note tracer deposits (magenta) around the CAV and pbv as well as in the leptomeninges. Br, brain. (C–E) Cavernous MLVs 1 and 2. Lymphatic vasculature (LYVE1+, green in C and D) and TUJ1+ cranial nerves (yellow in E) at coronal levels 1 and 2 (green arrowheads in C and D). Note MLVs close to the OVA-A555+ cavernous perisinusal space (C) and surrounding the foramen of iptgv (D). Cranial nerves were devoid of tracer deposits (E). VI, cranial nerve 6 (abducens); dotted line, skull border. (F–I) Cavernous MLVs 3. Dural veins (vWF, blue in F), lymphatic vasculature (LYVE1+, green in G and H), and cranial nerves (TUJ1+, yellow in I) at coronal (F, G, and I) and sagittal (H) level 3 (green arrowheads in F–H). MLVs contact the cavernous perisinusal space (G and H) and uptake tracer at the intersection of CAV with internal carotid arteries (white in G, magnified in inset). Cranial nerves were devoid of tracer deposits and MLVs (I). III, cranial nerve 3 (oculomotor); IV, cranial nerve 4 (trochlear); V, cranial nerve 5 (trigeminal); A, anterior; cc, carotid canal; D, dorsal; L, lateral; NP, nasopharyngeal cavity; P, posterior; V, ventral. Scale bar: 500 μm (B–I), 100 μm (inset in G).

MLV drainage from the caudal CAV. (A) Schematic of venous sinuses, veins, and internal carotid artery at the base of the brain. (B–I) Dashed lines indicate the level of coronal sections in B–I (inset in A), and lymphatics are numbered 1–3. BS, brainstem; bv, basilar vein; ica, internal carotid artery; ICAV, inter-CAV; ijv, internal jugular vein; IPETS, inferior petrosal sinus; iptgv, interpterygoid emissary vein; OB, olfactory bulb; pbv, posterior basal vein; pfv, posterior facial vein; SPETS, superior petrosal sinus. (B) Coronal view of the right CAV. OVA-A555 was injected into the Th-Lb spine 45 min before sacrifice, and the sample was labeled with antibodies recognizing blood endothelium markers CD31 and PDLX that label pituitary gland (PG) vessels as well as dura mater veins and sinuses (blue arrowheads). Blue dashed line, limits of CAV and iCAV. Note tracer deposits (magenta) around the CAV and pbv as well as in the leptomeninges. Br, brain. (C–E) Cavernous MLVs 1 and 2. Lymphatic vasculature (LYVE1+, green in C and D) and TUJ1+ cranial nerves (yellow in E) at coronal levels 1 and 2 (green arrowheads in C and D). Note MLVs close to the OVA-A555+ cavernous perisinusal space (C) and surrounding the foramen of iptgv (D). Cranial nerves were devoid of tracer deposits (E). VI, cranial nerve 6 (abducens); dotted line, skull border. (F–I) Cavernous MLVs 3. Dural veins (vWF, blue in F), lymphatic vasculature (LYVE1+, green in G and H), and cranial nerves (TUJ1+, yellow in I) at coronal (F, G, and I) and sagittal (H) level 3 (green arrowheads in F–H). MLVs contact the cavernous perisinusal space (G and H) and uptake tracer at the intersection of CAV with internal carotid arteries (white in G, magnified in inset). Cranial nerves were devoid of tracer deposits and MLVs (I). III, cranial nerve 3 (oculomotor); IV, cranial nerve 4 (trochlear); V, cranial nerve 5 (trigeminal); A, anterior; cc, carotid canal; D, dorsal; L, lateral; NP, nasopharyngeal cavity; P, posterior; V, ventral. Scale bar: 500 μm (B–I), 100 μm (inset in G).

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