Perisinusal distribution of CSF tracer and lymphatic uptake hotspots. (A and B) Coronal (A) and sagittal (B) views of the perisinusal distribution of tracer (OVA-A⁵⁵⁵, magenta, blue arrowheads) along the transversal sinus (TS, vWF⁺, blue). (C) LYVE1⁺ MLVs (green) and tracer deposits (magenta) in the rostral convergence of sinuses (RCS). Note tracer accumulation around RCS MLVs (blue arrowhead). OB, olfactory bulb. (D) Schematic lateral view of dural venous sinuses and localization of lymphatic uptake hotspots (1–3) in the caudal portion of CAV. bp, basilar plexus; Cx, cortex; ica, internal carotid artery; IPETS, inferior petrosal sinus; iptgv, interpterygoid emissary vein; pbv, posterior basal vein; SPETS, superior petrosal sinus. (E) Sagittal view of CD31⁺/PDLX⁺ blood vessels (red) and OVA-A⁵⁵⁵ deposits (magenta) around the pituitary gland (PG) and the CAV (blue dashed line). (F–I) Localization of lymphatic uptake hotspots (1–3) in the caudal portion of CAV: horizontal (F) or coronal (G–I) views of tracer deposits (magenta) and vWF⁺ dural sinuses and veins (blue). (I) Blood vessels (CD31⁺/PDLX⁺, orange) at the carotid canal (cc). Dotted line, skull border; ICAV, inter-CAV sinus. Scale bar: 200 μm (A–C); 500 μm (E–I).