Figure S4.
B cell–deficient Igh∆Jh/∆Jhmice show no behavioral abnormalities. All behavioral experiments (A–L) were performed with control Igh+/+ mice (blue) and Igh∆Jh/∆Jh (yellow) littermates (n ≥ 8 per genotype) at the age of 2–4 mo. (A) Rotarod assay. Latency to fall with a maximum acceleration of 40 revolutions per min on day 1–4 and 80 revolutions per min on day 5. (B) ErasmusLadder. Left: Mean time to cross the ladder per trial. Right: Mean frequency of missteps as percentage of total steps. (C and D) Three-chamber sociability and social novelty test. (C) Sociability test. Left: Total time in the chamber containing a cup with a “stranger 1” mouse, the center, or in the chamber with an empty cup (object). Right: Total time in the perimeter of the cup with stranger 1 mouse or the empty cup (object). (D) Social novelty test. Left: Total time in the chambers after introduction of a second “stranger 2” mouse. Right: Total time spent in the perimeter of the cup with stranger 1 or stranger 2. (E) Marble burying test. Percentage of marble surface area that is covered by bedding. (F) Self-grooming behavior. Total time spent grooming. (G) Open field test. Total time spent at the periphery or in the center of the arena. (H) Elevated plus maze. Time spent in the open and closed arms as well as in the center. (I) Hyperactivity profile. Distance covered during marble burying, self-grooming, and open field tests. (J) Water Y-maze assay. Percentage of correct trials during the acquisition, test, and reversal phases of the assay. During the reversal phase, the platform is placed in the opposite arm to assess reversal learning. (K and L) Eyeblink conditioning, as described in Video 3. (K) Percentage of conditioned responses (eye closure upon light pulse) across the entire experiment for CS-only trials. (L) Heatmaps displaying the mean amplitude of the conditioned response during the CS-only trials across the entire 10-d experiment for all mice of each genotype (white lines and the arrowhead denote the CS onset). All graphs show mean values with SEM, except for the violin plots (E), indicating median values with quartiles (n ≥ 8 per genotype). Two-way repeated measures ANOVA with Šídák’s multiple comparisons test (A, B, and K), two-way ANOVA with Tukey’s multiple comparisons test (C, D, G, and H), Mann–Whitney test (E), unpaired t test (F, I, and J); **, P < 0.01; ****, P < 0.0001. For detailed statistical information (A–K), see Table S6. Each dot (C–J) represents one mouse.

B cell–deficient Igh ∆Jh/∆Jh mice show no behavioral abnormalities. All behavioral experiments (A–L) were performed with control Igh+/+ mice (blue) and Igh∆Jh/∆Jh (yellow) littermates (n ≥ 8 per genotype) at the age of 2–4 mo. (A) Rotarod assay. Latency to fall with a maximum acceleration of 40 revolutions per min on day 1–4 and 80 revolutions per min on day 5. (B) ErasmusLadder. Left: Mean time to cross the ladder per trial. Right: Mean frequency of missteps as percentage of total steps. (C and D) Three-chamber sociability and social novelty test. (C) Sociability test. Left: Total time in the chamber containing a cup with a “stranger 1” mouse, the center, or in the chamber with an empty cup (object). Right: Total time in the perimeter of the cup with stranger 1 mouse or the empty cup (object). (D) Social novelty test. Left: Total time in the chambers after introduction of a second “stranger 2” mouse. Right: Total time spent in the perimeter of the cup with stranger 1 or stranger 2. (E) Marble burying test. Percentage of marble surface area that is covered by bedding. (F) Self-grooming behavior. Total time spent grooming. (G) Open field test. Total time spent at the periphery or in the center of the arena. (H) Elevated plus maze. Time spent in the open and closed arms as well as in the center. (I) Hyperactivity profile. Distance covered during marble burying, self-grooming, and open field tests. (J) Water Y-maze assay. Percentage of correct trials during the acquisition, test, and reversal phases of the assay. During the reversal phase, the platform is placed in the opposite arm to assess reversal learning. (K and L) Eyeblink conditioning, as described in Video 3. (K) Percentage of conditioned responses (eye closure upon light pulse) across the entire experiment for CS-only trials. (L) Heatmaps displaying the mean amplitude of the conditioned response during the CS-only trials across the entire 10-d experiment for all mice of each genotype (white lines and the arrowhead denote the CS onset). All graphs show mean values with SEM, except for the violin plots (E), indicating median values with quartiles (n ≥ 8 per genotype). Two-way repeated measures ANOVA with Šídák’s multiple comparisons test (A, B, and K), two-way ANOVA with Tukey’s multiple comparisons test (C, D, G, and H), Mann–Whitney test (E), unpaired t test (F, I, and J); **, P < 0.01; ****, P < 0.0001. For detailed statistical information (A–K), see Table S6. Each dot (C–J) represents one mouse.

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