Figure 8.
Lack of adipocyte FNIP1 improves systemic glucose homeostasis and alleviates hepatic steatosis in obese mice. (A) Left: Body weight of indicated male mice fed on HFD for 16 wk. n = 18–21 mice per group. Right: Food consumption per day. n = 6–12 mice per group. (B) Locomotor activity over 12-h light/dark cycle. n = 12–13 mice per group. (C) Oxygen consumption per hour during the light/dark cycle. n = 14–15 mice per group. (D) Left: GTT. Prior to studies, mice were fasted overnight. Right: Area under the curve for GTT is shown. n = 13–16 mice per group. (E) Left: ITT. Prior to studies, mice were fasted for 4 h. Right: Area under the curve for ITT is shown. n = 13–16 mice per group. (F) Representative Western blot analysis of Akt (S473) phosphorylation status relative to total AKT (T-AKT) in adipose and skeletal muscle 30 min after an intraperitoneal injection of insulin (2 U/kg) or saline in indicated male mice fed an HFD. All mice were fasted overnight before insulin or saline injection. (G) Quantification of p-AKT (S473)/T-AKT signal ratios. n = 3–4 mice per group. (H) Weights of iWAT normalized to body weight. n = 10–11 mice per group. (I) Top: Representative H&E staining of iWAT from indicated male mice fed an HFD. Scale bar: 20 μm. (Bottom) Cross-sectional areas of iWAT were measured by ImageJ. n = 5–7 mice per group. (J) Mitochondrial respiration rates were determined from the iWAT of the indicated male mice fed on HFD using succinate as substrate. n = 5–6 mice per group. (K) Top: Picture of liver from indicated male mice fed an HFD. Bottom: Weights of liver mass normalized to body weight. n = 10–11 mice per group. (L) Oil-Red (top) and H&E stain (bottom) of liver from HFD-fed male FNIP1 AKO mice and WT controls. Scale bar: 20 μm. n = 6–9 mice per group. (M) Liver triglyceride levels from HFD-fed male FNIP1 AKO mice and WT controls. n = 6–10 mice per group. (N) Top: Representative Western blot analysis of liver lysates for HFD-fed male mice of the indicated genotypes. Bottom: Quantification of the FASN/tubulin signal ratios. n = 4 mice per group. Values represent mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P value was determined using two-tailed unpaired Student’s t-test (A, H–K, M, and N), one-way ANOVA and Fisher’s LSD post-hoc test (G), or two-way ANOVA and Fisher’s LSD post-hoc test (B–E). Data are representative of two independent experiments (A–N). Source data are available for this figure: SourceData F8.

Lack of adipocyte FNIP1 improves systemic glucose homeostasis and alleviates hepatic steatosis in obese mice. (A) Left: Body weight of indicated male mice fed on HFD for 16 wk. n = 18–21 mice per group. Right: Food consumption per day. n = 6–12 mice per group. (B) Locomotor activity over 12-h light/dark cycle. n = 12–13 mice per group. (C) Oxygen consumption per hour during the light/dark cycle. n = 14–15 mice per group. (D) Left: GTT. Prior to studies, mice were fasted overnight. Right: Area under the curve for GTT is shown. n = 13–16 mice per group. (E) Left: ITT. Prior to studies, mice were fasted for 4 h. Right: Area under the curve for ITT is shown. n = 13–16 mice per group. (F) Representative Western blot analysis of Akt (S473) phosphorylation status relative to total AKT (T-AKT) in adipose and skeletal muscle 30 min after an intraperitoneal injection of insulin (2 U/kg) or saline in indicated male mice fed an HFD. All mice were fasted overnight before insulin or saline injection. (G) Quantification of p-AKT (S473)/T-AKT signal ratios. n = 3–4 mice per group. (H) Weights of iWAT normalized to body weight. n = 10–11 mice per group. (I) Top: Representative H&E staining of iWAT from indicated male mice fed an HFD. Scale bar: 20 μm. (Bottom) Cross-sectional areas of iWAT were measured by ImageJ. n = 5–7 mice per group. (J) Mitochondrial respiration rates were determined from the iWAT of the indicated male mice fed on HFD using succinate as substrate. n = 5–6 mice per group. (K) Top: Picture of liver from indicated male mice fed an HFD. Bottom: Weights of liver mass normalized to body weight. n = 10–11 mice per group. (L) Oil-Red (top) and H&E stain (bottom) of liver from HFD-fed male FNIP1 AKO mice and WT controls. Scale bar: 20 μm. n = 6–9 mice per group. (M) Liver triglyceride levels from HFD-fed male FNIP1 AKO mice and WT controls. n = 6–10 mice per group. (N) Top: Representative Western blot analysis of liver lysates for HFD-fed male mice of the indicated genotypes. Bottom: Quantification of the FASN/tubulin signal ratios. n = 4 mice per group. Values represent mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P value was determined using two-tailed unpaired Student’s t-test (A, H–K, M, and N), one-way ANOVA and Fisher’s LSD post-hoc test (G), or two-way ANOVA and Fisher’s LSD post-hoc test (B–E). Data are representative of two independent experiments (A–N). Source data are available for this figure: SourceData F8.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal